Review




Structured Review

Isu Abxis human breast tissue microarray
Overexpression of DNPH1 in human breast tumors (A) DNPH1 mRNA expression in various breast cancer subtypes was analyzed in published <t>microarray</t> data. Mean with standard deviation and statistical significance compared to normal breast tissue are indicated; ∗∗∗∗, p < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test). (B) High (top quartile) DNPH1 mRNA levels predict reduced survival. Data derived from published microarray experiments ; p < 0.0001 (log rank test). (C) DNPH1 protein expression in human breast cell lines assayed by Western blotting. (D) Examples of immunohistochemical staining with DNPH1 antibodies on matching normal and cancerous breast tissue. Scale bars, 0.1 mm. (E) DNPH1 protein expression was measured by immunohistochemistry in 20 matching normal and cancerous human breast tissues; two-tailed, paired t test.
Human Breast Tissue Microarray, supplied by Isu Abxis, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+microarray/breast+human+microarray+tissue/pmc12990376-18-0-5
Average 86 stars, based on 1 article reviews
human breast tissue microarray - by Bioz Stars, 2026-10
86/100 stars

Images

1) Product Images from "Promotion of breast cancer by the DNPH1 enzyme"

Article Title: Promotion of breast cancer by the DNPH1 enzyme

Journal: iScience

doi: 10.1016/j.isci.2026.115227

Overexpression of DNPH1 in human breast tumors (A) DNPH1 mRNA expression in various breast cancer subtypes was analyzed in published microarray data. Mean with standard deviation and statistical significance compared to normal breast tissue are indicated; ∗∗∗∗, p < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test). (B) High (top quartile) DNPH1 mRNA levels predict reduced survival. Data derived from published microarray experiments ; p < 0.0001 (log rank test). (C) DNPH1 protein expression in human breast cell lines assayed by Western blotting. (D) Examples of immunohistochemical staining with DNPH1 antibodies on matching normal and cancerous breast tissue. Scale bars, 0.1 mm. (E) DNPH1 protein expression was measured by immunohistochemistry in 20 matching normal and cancerous human breast tissues; two-tailed, paired t test.
Figure Legend Snippet: Overexpression of DNPH1 in human breast tumors (A) DNPH1 mRNA expression in various breast cancer subtypes was analyzed in published microarray data. Mean with standard deviation and statistical significance compared to normal breast tissue are indicated; ∗∗∗∗, p < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test). (B) High (top quartile) DNPH1 mRNA levels predict reduced survival. Data derived from published microarray experiments ; p < 0.0001 (log rank test). (C) DNPH1 protein expression in human breast cell lines assayed by Western blotting. (D) Examples of immunohistochemical staining with DNPH1 antibodies on matching normal and cancerous breast tissue. Scale bars, 0.1 mm. (E) DNPH1 protein expression was measured by immunohistochemistry in 20 matching normal and cancerous human breast tissues; two-tailed, paired t test.

Techniques Used: Over Expression, Expressing, Microarray, Standard Deviation, Derivative Assay, Western Blot, Immunohistochemical staining, Staining, Immunohistochemistry, Two Tailed Test

Related Articles

Expressing:

Article Title: USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2
Article Snippet: .. The expression patterns of USP32 and SLC35F2 in human breast (n = 21), colon (n = 32), and lung cancers (n = 32) obtained from ISU Abxis tissue microarray were subjected to immunohistochemistry staining. ..

Article Title: KRAS Activation in Gastric Adenocarcinoma Stimulates Epithelial-to-Mesenchymal Transition to Cancer Stem–Like Cells and Promotes Metastasis
Article Snippet: .. CD44 expression and RTK-RAS activation is associated with worse survival in patients with resectable GA To further examine the role of CSCs and RTK-RAS activation in human GAs, we next assessed expression of CD44 and activation of MEK and ERK by immunostaining a commercially available tissue microarray containing 40 human GAs and 8 human normal gastric tissues (A209 II, ISU ABXIS, Seoul, Republic of Korea). ..

Article Title: Ubiquitin Specific Protease 29 Functions as an Oncogene Promoting Tumorigenesis in Colorectal Carcinoma
Article Snippet: .. To investigate the expression of USP29 in human colon cancer tissues, we performed an immunostaining analysis in a tissue microarray (TMA) containing colon cancer tissues ( n = 32) and corresponding normal tissues ( n = 32) obtained from the ISU Abxis TMA. ..

Microarray:

Article Title: USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2
Article Snippet: .. The expression patterns of USP32 and SLC35F2 in human breast (n = 21), colon (n = 32), and lung cancers (n = 32) obtained from ISU Abxis tissue microarray were subjected to immunohistochemistry staining. ..

Article Title: Altered expression of the RON receptor tyrosine kinase in various epithelial cancers and its contribution to tumourigenic phenotypes in thyroid cancer cells.
Article Snippet: Aberrant expression of the RON receptor tyrosine kinase has been implicated in the pathogenesis of epithelial tumours.. The aim of this study was to determine RON expression in various normal epithelial cells and their corresponding tumours by immunohistochemistry.. The role of RON in regulating tumourigenic phenotypes was also studied using thyroid cancer cells as a model. RON was almost exclusively expressed at variable levels in normal epithelial cells from the digestive track, lung, kidney, pancreas, liver, breast, bladder, skin, and others.

Article Title: KRAS Activation in Gastric Adenocarcinoma Stimulates Epithelial-to-Mesenchymal Transition to Cancer Stem–Like Cells and Promotes Metastasis
Article Snippet: .. CD44 expression and RTK-RAS activation is associated with worse survival in patients with resectable GA To further examine the role of CSCs and RTK-RAS activation in human GAs, we next assessed expression of CD44 and activation of MEK and ERK by immunostaining a commercially available tissue microarray containing 40 human GAs and 8 human normal gastric tissues (A209 II, ISU ABXIS, Seoul, Republic of Korea). ..

Article Title: Methods for performing direct enzymatic reactions involving nucleic acid molecules
Article Snippet: .. To verify the possibility of the direct PCR using slide tissues, a spot was extracted from the tissue microarray (ISU Abxis, Inc.) and subjected directly to PCR. ..

Article Title: Ubiquitin Specific Protease 29 Functions as an Oncogene Promoting Tumorigenesis in Colorectal Carcinoma
Article Snippet: .. To investigate the expression of USP29 in human colon cancer tissues, we performed an immunostaining analysis in a tissue microarray (TMA) containing colon cancer tissues ( n = 32) and corresponding normal tissues ( n = 32) obtained from the ISU Abxis TMA. ..

Article Title: Microsatellite instability in endometrioid type endometrial adenocarcinoma is associated with poor prognostic indicators.
Article Snippet: Microsatellite instability (MSI) has been reported in 25% to 45% of sporadic endometrial carcinoma.. The clinicopathologic and molecular characteristics of MSI-high phenotype in colorectal and gastric carcinomas have been widely investigated; however, the clinicopathologic impact of MSI on endometrial carcinomas remained unclear.. This study was performed to determine the clinicopathologic and molecular significance of MSI in endometrial carcinomas.

Immunohistochemistry:

Article Title: USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2
Article Snippet: .. The expression patterns of USP32 and SLC35F2 in human breast (n = 21), colon (n = 32), and lung cancers (n = 32) obtained from ISU Abxis tissue microarray were subjected to immunohistochemistry staining. ..

Article Title: Altered expression of the RON receptor tyrosine kinase in various epithelial cancers and its contribution to tumourigenic phenotypes in thyroid cancer cells.
Article Snippet: Aberrant expression of the RON receptor tyrosine kinase has been implicated in the pathogenesis of epithelial tumours.. The aim of this study was to determine RON expression in various normal epithelial cells and their corresponding tumours by immunohistochemistry.. The role of RON in regulating tumourigenic phenotypes was also studied using thyroid cancer cells as a model. RON was almost exclusively expressed at variable levels in normal epithelial cells from the digestive track, lung, kidney, pancreas, liver, breast, bladder, skin, and others.

Staining:

Article Title: USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2
Article Snippet: .. The expression patterns of USP32 and SLC35F2 in human breast (n = 21), colon (n = 32), and lung cancers (n = 32) obtained from ISU Abxis tissue microarray were subjected to immunohistochemistry staining. ..

Article Title: Altered expression of the RON receptor tyrosine kinase in various epithelial cancers and its contribution to tumourigenic phenotypes in thyroid cancer cells.
Article Snippet: Aberrant expression of the RON receptor tyrosine kinase has been implicated in the pathogenesis of epithelial tumours.. The aim of this study was to determine RON expression in various normal epithelial cells and their corresponding tumours by immunohistochemistry.. The role of RON in regulating tumourigenic phenotypes was also studied using thyroid cancer cells as a model. RON was almost exclusively expressed at variable levels in normal epithelial cells from the digestive track, lung, kidney, pancreas, liver, breast, bladder, skin, and others.

Activation Assay:

Article Title: KRAS Activation in Gastric Adenocarcinoma Stimulates Epithelial-to-Mesenchymal Transition to Cancer Stem–Like Cells and Promotes Metastasis
Article Snippet: .. CD44 expression and RTK-RAS activation is associated with worse survival in patients with resectable GA To further examine the role of CSCs and RTK-RAS activation in human GAs, we next assessed expression of CD44 and activation of MEK and ERK by immunostaining a commercially available tissue microarray containing 40 human GAs and 8 human normal gastric tissues (A209 II, ISU ABXIS, Seoul, Republic of Korea). ..

Immunostaining:

Article Title: KRAS Activation in Gastric Adenocarcinoma Stimulates Epithelial-to-Mesenchymal Transition to Cancer Stem–Like Cells and Promotes Metastasis
Article Snippet: .. CD44 expression and RTK-RAS activation is associated with worse survival in patients with resectable GA To further examine the role of CSCs and RTK-RAS activation in human GAs, we next assessed expression of CD44 and activation of MEK and ERK by immunostaining a commercially available tissue microarray containing 40 human GAs and 8 human normal gastric tissues (A209 II, ISU ABXIS, Seoul, Republic of Korea). ..

Article Title: Ubiquitin Specific Protease 29 Functions as an Oncogene Promoting Tumorigenesis in Colorectal Carcinoma
Article Snippet: .. To investigate the expression of USP29 in human colon cancer tissues, we performed an immunostaining analysis in a tissue microarray (TMA) containing colon cancer tissues ( n = 32) and corresponding normal tissues ( n = 32) obtained from the ISU Abxis TMA. ..

Construct:

Article Title: The Role and Regulatory Mechanism of 14-3-3 Sigma in Human Breast Cancer
Article Snippet: .. A tissue microarray was constructed by ISU Abxis Co. (Seoul, Korea) from the paraffin-embedded blocks of 220 breast cancer cases. ..

Article Title: Microsatellite instability in endometrioid type endometrial adenocarcinoma is associated with poor prognostic indicators.
Article Snippet: Microsatellite instability (MSI) has been reported in 25% to 45% of sporadic endometrial carcinoma.. The clinicopathologic and molecular characteristics of MSI-high phenotype in colorectal and gastric carcinomas have been widely investigated; however, the clinicopathologic impact of MSI on endometrial carcinomas remained unclear.. This study was performed to determine the clinicopathologic and molecular significance of MSI in endometrial carcinomas.

Polymerase Chain Reaction:

Article Title: Methods for performing direct enzymatic reactions involving nucleic acid molecules
Article Snippet: .. To verify the possibility of the direct PCR using slide tissues, a spot was extracted from the tissue microarray (ISU Abxis, Inc.) and subjected directly to PCR. ..



Similar Products

86
Sakura Finetek tissue tek quick ray microarray system
Tissue Tek Quick Ray Microarray System, supplied by Sakura Finetek, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+microarray/5+console+embedding+system+tectm+tek+tissue+tissue/pm42321016-53-5-9
Average 86 stars, based on 1 article reviews
tissue tek quick ray microarray system - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

86
Human Protein Atlas hpa tissue microarray data v22 16
Hpa Tissue Microarray Data V22 16, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+microarray/data+microarray+tissue/pm42252507-66-30-27
Average 86 stars, based on 1 article reviews
hpa tissue microarray data v22 16 - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

86
Human Protein Atlas tissue microarray data
Tissue Microarray Data, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+microarray/microarrays+tissue/pm42265770-240-11-16
Average 86 stars, based on 1 article reviews
tissue microarray data - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

86
Servicebio Inc clinical specimens hcc tissue microarrays
Clinical Specimens Hcc Tissue Microarrays, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+microarray/microarrays+tissue+tma/pm42259775-114-3-11
Average 86 stars, based on 1 article reviews
clinical specimens hcc tissue microarrays - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

86
Servicebio Inc tissue microarray
Tissue Microarray, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+microarray/microarray+tissue/pm42123694-178-1-10
Average 86 stars, based on 1 article reviews
tissue microarray - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

86
Isu Abxis human breast tissue microarray
Overexpression of DNPH1 in human breast tumors (A) DNPH1 mRNA expression in various breast cancer subtypes was analyzed in published <t>microarray</t> data. Mean with standard deviation and statistical significance compared to normal breast tissue are indicated; ∗∗∗∗, p < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test). (B) High (top quartile) DNPH1 mRNA levels predict reduced survival. Data derived from published microarray experiments ; p < 0.0001 (log rank test). (C) DNPH1 protein expression in human breast cell lines assayed by Western blotting. (D) Examples of immunohistochemical staining with DNPH1 antibodies on matching normal and cancerous breast tissue. Scale bars, 0.1 mm. (E) DNPH1 protein expression was measured by immunohistochemistry in 20 matching normal and cancerous human breast tissues; two-tailed, paired t test.
Human Breast Tissue Microarray, supplied by Isu Abxis, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+microarray/breast+human+microarray+tissue/pmc12990376-18-0-5
Average 86 stars, based on 1 article reviews
human breast tissue microarray - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

86
Biomax Inc brain tissue microarrays
Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue <t>microarrays</t> with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.
Brain Tissue Microarrays, supplied by Biomax Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+microarray/microarrays+tissue/pmc13052156-61-2-17
Average 86 stars, based on 1 article reviews
brain tissue microarrays - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

86
Biomax Inc normal brain tissue microarrays
Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue <t>microarrays</t> with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.
Normal Brain Tissue Microarrays, supplied by Biomax Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+microarray/microarrays+tissue/pmc13052156-395-6-11
Average 86 stars, based on 1 article reviews
normal brain tissue microarrays - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

86
Human Protein Atlas tissue microarray based staining data
Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue <t>microarrays</t> with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.
Tissue Microarray Based Staining Data, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+microarray/data+microarray+tissue/pm41898689-164-18-22
Average 86 stars, based on 1 article reviews
tissue microarray based staining data - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

Image Search Results


Overexpression of DNPH1 in human breast tumors (A) DNPH1 mRNA expression in various breast cancer subtypes was analyzed in published microarray data. Mean with standard deviation and statistical significance compared to normal breast tissue are indicated; ∗∗∗∗, p < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test). (B) High (top quartile) DNPH1 mRNA levels predict reduced survival. Data derived from published microarray experiments ; p < 0.0001 (log rank test). (C) DNPH1 protein expression in human breast cell lines assayed by Western blotting. (D) Examples of immunohistochemical staining with DNPH1 antibodies on matching normal and cancerous breast tissue. Scale bars, 0.1 mm. (E) DNPH1 protein expression was measured by immunohistochemistry in 20 matching normal and cancerous human breast tissues; two-tailed, paired t test.

Journal: iScience

Article Title: Promotion of breast cancer by the DNPH1 enzyme

doi: 10.1016/j.isci.2026.115227

Figure Lengend Snippet: Overexpression of DNPH1 in human breast tumors (A) DNPH1 mRNA expression in various breast cancer subtypes was analyzed in published microarray data. Mean with standard deviation and statistical significance compared to normal breast tissue are indicated; ∗∗∗∗, p < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test). (B) High (top quartile) DNPH1 mRNA levels predict reduced survival. Data derived from published microarray experiments ; p < 0.0001 (log rank test). (C) DNPH1 protein expression in human breast cell lines assayed by Western blotting. (D) Examples of immunohistochemical staining with DNPH1 antibodies on matching normal and cancerous breast tissue. Scale bars, 0.1 mm. (E) DNPH1 protein expression was measured by immunohistochemistry in 20 matching normal and cancerous human breast tissues; two-tailed, paired t test.

Article Snippet: Human breast tissue microarray , ISU Abxis , A312.

Techniques: Over Expression, Expressing, Microarray, Standard Deviation, Derivative Assay, Western Blot, Immunohistochemical staining, Staining, Immunohistochemistry, Two Tailed Test

Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue microarrays with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.

Journal: The Journal of Biological Chemistry

Article Title: Inhibition of cell surface GRP78 on brain tumors reverses drug resistance and stops cancer stem cell expansion

doi: 10.1016/j.jbc.2026.111146

Figure Lengend Snippet: Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue microarrays with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.

Article Snippet: A, three brain tissue microarrays with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers.

Techniques: Staining, Microarray

Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue microarrays with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.

Journal: The Journal of Biological Chemistry

Article Title: Inhibition of cell surface GRP78 on brain tumors reverses drug resistance and stops cancer stem cell expansion

doi: 10.1016/j.jbc.2026.111146

Figure Lengend Snippet: Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue microarrays with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.

Article Snippet: Briefly, three human brain tumors and normal brain tissue microarrays (T174c; Biomax. us) were deparaffinized with xylene and washed with PBS.

Techniques: Staining, Microarray